The Journal of Cutaneous and Genito-Urinary Disease, Vol. 17 : January, 1899 (Classic Reprint) by Unknown Author
Excerpt from The Journal of Cutaneous and Genito-Urinary Disease, Vol. 17: January, 1899
The method of procedure adopted was as follows: an observation of the case clinically; an examination of the hair or the scale microscopically; a planting of the suspected tissues; and in many cases a microscopical examination of the culture. All hairs and scales were examined at first unstained, and later, in some instances, stained specimens were prepared, but I do not believe in the latter method, for by this artificial process only the living spores are colored and the dead ones remain in obscurity. The result is a picture far from the true one - a picture which does not present the plant as it actually exists. Several methods of staining were tried but that recommended by Adamson of London was finally adopted as the most satisfactory.
After experimenting with many different artificial media for the cultivation of the plant I adopted the "milieu-d'epreuve" of Sabouraud as the one soil most favorable to the local varieties of ringworm, and although many variations were made in the proportions of the ingredients, nevertheless, in the end I returned to the original formula and used it during the greater part of my work. Success seldom came to me in my cultures, for out of nearly three hundred inoculations less than fifteen percent, produced favorable results. This percentage refers to distinct cases, for when once an individual plant showed itself capable of artificial reproduction it was almost always possible to obtain sub-cultures ad libitum. These sub-cultures almost always retained the most minute peculiarities of the parent stock and to my mind afforded one of the prettiest demonstrations of the plurality of the ringworm fungi. The maltose of several different manufacturers was tried during the three years, and I found that the resulting cultures varied only slightly - the growth of the microsporon Audouini being white when sown on the maltose of Lehn and Fink of New York and buff when planted on that of Grubler of Leipsig. This was the most striking variation noted, and one can see that this was not as important as those found by European investigators in their studies.
The temperature at which the cultures were left to grow was found to influence their characteristics to a considerable extent and after many trials 30 centigrade was decided upon as the one best fitted to produce a rapid and luxuriant growth. As to the reaction of the medium it was found that the more acid it was the more contaminations flourished, while with too much alkali present the vigor of the ringworm was threatened. Therefore, a slightly alkaline reaction was found to be the most favorable.
During my second-year's work I discovered, to my temporary disadvantage, an excellent means of checking further growth in a culture and also of rendering hairs and scales infected with ringworm sterile.
About the Publisher
Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com
This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works."
Read online The Journal of Cutaneous and Genito-Urinary Disease, Vol. 17 : January, 1899 (Classic Reprint)
Buy The Journal of Cutaneous and Genito-Urinary Disease, Vol. 17 : January, 1899 (Classic Reprint)
Download and read The Journal of Cutaneous and Genito-Urinary Disease, Vol. 17 : January, 1899 (Classic Reprint) ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent
Download to iPad/iPhone/iOS, B&N nook The Journal of Cutaneous and Genito-Urinary Disease, Vol. 17 : January, 1899 (Classic Reprint)
